Daily Programme

 Download a pdf version here.

Monday, July 9

Tuesday, July 10

Wednesday, July 11

Thursday, July 12

Friday, July 13

Image Formation

&

Transmitted-light Techniques I

Transmitted-light Technques II

&

Introduction to Fluorescence Microscopy

Fluorescence Microscopy: Experimental Design

&

 Image Processing and Analysis

Specialized Techniques in Fluorescence Microscopy

Advanced Techniques

Introductory Talk

Light-matter interactions

Basic optics and image    formation

Brightfield and phase  contrast

Objectives and  corrections, resolution and sampling frequency

 

 

Polarization and Differential interference contrast (DIC) Microscopy

Fluorescence and fluorescent markers

Anatomy of a fluorescence microscope

-      excitation sources

-      filters

-      objective lenses

-      detectors

 

Immunofluorescence

Multi-colour experiments

Digital images

Intoduction to image processing and analysis

Quantitative imaging

The ethics of image manipulation

 

 

Confocal laser scanning microscopy

Multiphoton microscopy

Spinning disk confocal microscopy 

Superresolution microscopy

Deblurring and deconvolution

Which microscopy technique is right for your application? Case studies

Live cell imaging

The perils of co-localization measurements

FRET

Advanced image processing – tracking, 3D rendering, 3D measurements

Open discussion of any aspects of the course.

 

Labs (Full Course Only)

Kohler alignment & image and aperature planes

Phase contrast and visible dyes

Labs (Full Course Only)

DIC

Fluorescence

Labs (Full Course Only)

Image processing and analysis

Multi-colour and cameras

Labs (Full Course Only)

Rotate to different 3D platforms

Labs (Full Course Only)

Live Cell

Advanced image processing

 

Monday, July 16

Tuesday, July 17

Wednesday, July 18

Thursday, July 19

Friday, July 20

Protein Dynamics and Interactions #1

Protein Dynamics and Interactions #2

Nanoscopic (Superresolution) Imaging

In Vivo Imaging

Correlative Light Electron Microsopy (CLEM)

 

Fluorescent Proteins

Photobleaching Techniques

Colocalization

FRET

FLIM-FRET

 

 Fluorescence cross correlation spectroscopy (FCCS)

Image correlation microscopy

Number versus brightness analysis

 

Introductory Talk

Stimulated Emission Depletion (STED)

Structured Illumination Microscopy (SIM)

Single Molecule Localization Techniques

Labels for Nanoscopy

 

Live Cell Imaging

Practical tips for In Vivo avian embryo imaging 

Light sheet microscopy

High speed multi-colour spinning disk confocal microscopy

Multiphoton

 

 

Introduction to EM, SEM, TEM, Cryo

Practical aspects of CLEM

CLEM probes

Applications of CLEM

Open Discussion

Super-Resolution or EM?

Spatial co-localization vs functional interaction?

 

Labs (Full Course Only)

TBA

Labs (Full Course Only)

TBA

Labs (Full Course Only)

TBA

Labs (Full Course Only)

TBA

Labs (Full Course Only)

TBA